Fig. 4

Recognition of predicted epitopes by CD8 ⁺ T cells. Epitopes for recurrent mutations that have been identified in silico to bind to HLA-A*02:01 using our pipeline were synthesized and used for immunization of human TCR transgenic ABabDII mice. Examples (RAC1:P29S and TRRAP:S722F) of ex vivo ICS analysis of mutant peptide immunized ABabDII mice 7 days after the last immunization are shown. Polyclonal stimulation with CD3/CD28 dynabeads was used as positive control, stimulation with an irrelevant peptide served as negative control (data not shown)