Fig. 4From: Integrated transcriptome and proteome analyses identify novel regulatory network of nucleus pulposus cells in intervertebral disc degenerationVerification of the expression of candidate genes in normal (IVD) and degenerated (IDD) NP primary cells. a The expression of TNFAIP6, CHI3L1, KRT19, DPT, COL6A2, and COL11A2 were examined in normal (IVD, n = 6) and degenerated (IDD, n = 6) NP tissues using immunohistochemistry. b The quantifications of the immunohistochemistry analysis were performed to show the relative expression level of the candidate genes. c The RNA expression level of these candidate genes in normal (IVD, n = 3) and degenerated (IDD, n = 3) NP primary cells were assessed using real-time PCR method. d The RNA expression level of these candidate genes in normal (NC, n = 3) and IL-1β (50 ng/ml, resembles the degeneration process in vitro, n = 3) treated NP primary cells were assessed using real-time PCR method. RNA level of GAPDH was used as internal reference. All Data are represented as mean ± SD, **p < 0.01Back to article page