- Case report
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Brainstem intraparenchymal schwannoma with genetic analysis: a case report and literature review
BMC Medical Genomics volume 14, Article number: 205 (2021)
Schwannomas are neoplasms that typically arise from the myelin sheath of peripheral nerves and rarely originate within the brain parenchyma. Some case reports present schwannomas arising from the brainstem, but regrowth of the tumor and the efficacy of postoperative irradiation have not been examined. In addition, the genetic background of schwannomas arising from the brainstem has not been investigated.
A 21-year-old male presented with diplopia, dysphagia, and left-sided hemiparesis, dysesthesia, and ataxia. Intracranial imaging showed a heterogeneous mass with a cystic lesion in the pontomedullary junction. Since the tumor caused obstructive hydrocephalus, the patient underwent subtotal tumor resection. A histopathologic evaluation aided a diagnosis of brainstem intraparenchymal schwannoma. Gradual postoperative mass regrowth was recognized. Three-dimensional conformal radiotherapy was performed on the residual mass and surgical cavity. No tumor regrowth was observed 4 years after surgery. To investigate the genetic background of the tumor, target sequences for 36 genes, including NF2, SMARCB1, and LZTR1, and microsatellite analysis for loss of 22q did not show any somatic variants or 22q loss.
We suggest that brainstem schwannomas might differ from conventional schwannomas in their genetic background.
Schwannomas arise from the myelin sheath of peripheral nerves and account for 8–10% of all intracranial tumors . They are usually encountered in extra‑axial locations. Intra‑axial schwannomas are rare and arouse significant histopathological curiosity because they account for less than 1% of intracranial schwannomas. They have been reported in periventricular regions and in the cerebellum, but brainstem intraparenchymal lesions are rarely reported [2,3,4]. In addition, no reports have genetically examined brainstem intraparenchymal schwannomas. In this study, we report a case of pontomedullary intraparenchymal schwannoma and discuss its histological origins and genetic background.
History and examination
A 21-year-old male without any notable past medical history presented to our hospital with numbness in the left upper extremity. Symptoms emerged a few months before the patient presented at our hospital and had been gradually deteriorating. Upon admission, a neurological examination revealed bilateral abducent palsy with partial impairment of adduction in the right eye when the patient looked to the left (one and a half syndrome), dysphagia, and left-sided hemiparesis, dysesthesia, and ataxia. His family history, including schwannomas and meningiomas, was unremarkable. Brain magnetic resonance imaging (MRI) showed a 4-cm mass in the dorsal pontomedullary area adjacent to the fourth ventricle (Fig. 1a–f). The contrast-enhanced mass was accompanied by a large cyst behind the solid component. No contrast enhancement was observed on the cyst wall. There were no other lesions such as vestibular schwannoma or spinal schwannoma in this patient. With 18F-fluorodeoxyglucose positron emission tomography, uptake in the lesion was non-significant, indicating minimal or no malignancy. A thorough survey of malignancy in the trunk did not reveal any lesions. Preoperative diagnostic angiography did not show tumor staining, which suggested a differential diagnosis of pilocytic astrocytoma. The fourth ventricle partially collapsed due to the mass effect, and signs of intracranial hypertension caused by obstructive hydrocephalus gradually emerged; thus, urgent tumor resection was planned.
The location of the motor and sensory tracts was confirmed by tractography with diffusion tensor imaging and Q-ball imaging prior to surgery. The motor and sensory tract was anteriorly and laterally compressed by the tumor, respectively. An approach from the posterior side was planned, based on the results of tractography (Fig. 2a, b).
A midline suboccipital craniotomy with a transcerebellomedullary fissure approach was performed. Immediately after the craniotomy, an external ventricular drain was inserted. The posterior wall of the cystic component was visible along the midline of bilateral facial colliculi (Fig. 2c). After suction of the yellowish liquid from the cyst, the anterior solid component of the tumor was identified.
Bradycardia and hypotension occurred during removal of the ventrocaudal component of the tumor, which might have been due to stimulation of the dorsal nucleus of the vagus nerve. Thus, the procedure was terminated, resulting in incomplete removal of the tumor.
A tracheotomy was performed immediately after tumor removal to prevent postoperative respiratory adverse events. Obstructive hydrocephalus resolved after tumor resection, and external ventricular drainage was removed on postoperative day (POD) 10. The ventrocaudal residual mass was followed up with gadolinium-enhanced MRI (Fig. 1g). The image on POD 29 showed slight enlargement of the contrast-enhanced area. The radiographical change was initially regarded as a postoperative inflammatory reaction, but MRI performed on POD 43 showed apparent growth of the solid component (Fig. 1h). Local fractionated irradiation was initiated at 50 Gy/25 Fr from POD 50. Subsequent follow-up MRI demonstrated no tumor regrowth. Three months after surgery, the tracheostomy was closed, and the patient was transferred to a rehabilitation facility. Left-sided facial paralysis and abducent palsy persisted even after discharge from the facility, but left-sided hemiparesis improved. Currently, no tumor regrowth has been observed 4 years after surgery (Fig. 1i).
Pathological and immunohistochemical investigation
A representative formalin-fixed paraffin-embedded tissue block was chosen for hematoxylin–eosin staining and immunohistochemistry, and sections with 3-µm thickness were prepared. Immunohistochemistry was performed with Ventana Benchmark (Roche, Basel, Switzerland) as the manufacturer's protocols. Primary antibodies used were as follows: anti-S100 (rabbit polyclonal, prediluted, Roche), anti-epithelial membrane antigen (EMA) (mouse monoclonal, clone E29, prediluted Roche), anti-glial fibrillary protein (GFAP) (rabbit polyclonal, Nichirei, Tokyo, Japan), Ki-67 (rabbit monoclonal, clone 30-9, Roche). S100 was evaluated in nuclei and cytoplasm. EMA was evaluated at the cell membrane. GFAP was evaluated in cytoplasm. Ki-67 labeling index was calculated as percent positive tumor nuclei.
To reveal the genetic background of the tumor, target sequences were performed on all exons in the 36 selected genes from the tumor and germline DNA, including NF2, SMARCB1, and LZTR1, using HaloPlexHS targeted capture (Agilent Technologies, Santa Clara, USA). Targeted genes were NF2, SMARCB1, LZTR1, ALPK2, APC, ARID1A, ARID1B, BAP1, BRAF, BRCA2, CABIN1, CAST, CDKN2A, CDKN2B, COQ6, DDR1, EPB41L3, GLI1, MEN1, MUTYH, PIK3CA, PTCH1, PTEN, SDHD, SHH, SMARCE1, SMO, SUFU, TAB3, TERT, TP53, TRAF7, TSC1, TSC2, and WRN. These genes are reported as pertinent in schwannomas and other related tumors [5, 6].
Sequencing data was generated using the Illumina HiSeq 2000 sequencer and the 100-base pair paired-end sequencing protocol across rapid flow cell lanes. An analysis of the number of reads of each amplicon in the HaloplexHS data was performed with a mean coverage of 119 × with barcodes for the tumor sample and 278 × with barcodes for the blood sample. Somatic variants were initially called following GATK best practice using Mutect2, and false positives were filtered out by GATK FilterMutectCalls. On the other hand, germline variants were initially called following GATK best practice using Haplotypecaller, and potential false positives were filtered out by FilterVariantTranches after CNNScoreVariants with default parameters. Funcotator including ClinVar database was applied to annotate the remained variants. Sequencing reads were aligned to the human genome (hg19). Detailed conditions will be provided upon a reasonable request.
Microsatellite analysis was also performed to detect 22q loss. Five microsatellite polymorphic markers flanking NF2 were selected from the Genome Database and included D22S268, D22S1163, D22S929, D22S280, and D22S282. Detailed conditions of this analysis will be provided upon a reasonable request.
The final pathology report suggested brainstem intraparenchymal schwannoma. The specimen had typical characteristics of a schwannoma, such as Antoni A and B regions (Fig. 3a, b). The Ki-67 labeling index was 5%, but some components had a Ki-67 labeling index of 10–20% (Fig. 3c). Immunohistochemical staining for S100 protein was diffusely and intensely positive (Fig. 3d), whereas GFAP and EMA were negative in the tumor cells.
With regard to somatic variants, 29 variants were initially called, but 27 variants were filtered out as false positives by GATK FilterMutectCalls, and the remaining two indels were within the introns or the intragenic regions. As for germline mutations, 406 variants were initially called, and 10 of those were filtered out by FilterVariantTranches after CNNScoreVariants with default parameters. Of the remaining 396 variants, 69 were indels within the introns or the untranslated regions. None of the remining 327 single-nucleotide variants were pathogenic, according to the analysis by Funcotator including ClinVar database. In summary, no pathogenic variants were detected in NF2, SMARCB1, LZTR1, or the other targeted genes. The 22q loss was also not detected (Fig. 4). Regarding somatic copy number variants and exon deletions, reliable results could not be obtained.
We experienced the first case of brainstem schwannoma recurrence after resection, which was successfully controlled by irradiation. Intraparenchymal schwannomas are extremely rare, and few have been reported in the brainstem region. Some researchers have reported rapid regrowth after tumor resection [7,8,9], but there are no reports of postoperative relapse of brainstem schwannoma.
Only 14 case reports of brainstem intraparenchymal schwannoma have been published (Table 1) [2,3,4, 10,11,12,13,14,15,16,17,18,19]. According to a literature review by Gao et al.  of the 84 cases of intraparenchymal schwannoma, 23 cases occurred in the posterior fossa, and 9 in the brainstem. Interestingly, in almost all cases, the solid component was located anteriorly, and the cystic component was situated posteriorly. Furthermore, there was a bimodal patient distribution of adolescents and middle-aged individuals. Despite difficulty in the surgical approach and multiple adjacent critical structures, prognosis for patients with brainstem intraparenchymal schwannoma is relatively favorable. Moreover, prognosis for patients with overall intraparenchymal schwannoma is generally favorable . However, three previous reports demonstrated rapid relapses [7,8,9]. The Ki-67 index is not mentioned in these reports. In our case, the Ki-67 index was 10–20% in the mitotic area. Given this pathological report, past malignant cases may have had similar findings. That is, we need to stay vigilant to detect postoperative recurrence if the pathology report indicates high Ki-67 index.
Some researchers speculate that specific soft membrane cells have the potential to transform into Schwann cells, which are the origin of intracerebral schwannomas . Although the development of intraparenchymal schwannomas remains unclear, many researchers believe that intraparenchymal schwannomas originate from the subarachnoid space and peripheral venous plexus . In fact, most intraparenchymal schwannomas are located in the superficial zone, such as on the cortical surface or in the paraventricular space. Neural crest cells directly differentiate into Schwann cell precursors that further transform into immature Schwann cells, which, at the stage of fetal birth, differentiate into demyelinated Schwann cells [22,23,24,25,26]. Hence, neural crest cells might be trapped in the brain parenchyma during embryonic development can give rise to intraparenchymal schwannomas.
Our genetic analysis of the tumor did not show any genetic alterations pertinent to schwannomas including NF2 mutation or 22q loss of heterozygosity, whereas the genetic alteration of NF2 gene occurs in 50 to 85% of sporadic vestibular schwannomas [5, 27,28,29,30,31]. To our knowledge, we described the result of genetic examination performed on brainstem schwannoma for the first time. Brainstem schwannomas may differ from conventional schwannomas (e.g., vestibular and spinal schwannoma) in terms of their genetic background, though further similar studies are essential to confirm this hypothesis.
We could not obtain reliable results on exonic deletions in the targeted genes and did not perform further investigation for the exonic deletions (e.g., multiplex ligation-dependent probe amplification). To clarify the genetic background of brainstem schwannomas, genome-wide comprehensive genetic analysis is essential. In addition, epigenetic analysis could also be important. Multiple studies on vestibular schwannomas showed that hypermethylation of the NF2 locus, which leads to decreased expression of NF2, is a rare phenomenon [32, 33]. However, it does not necessarily mean NF2 promoter methylation rarely occurs in brainstem schwannomas. Furthermore, in a previous report, genome-wide methylation profiling of vestibular and spinal schwannoma revealed distinct molecular subgroups of schwannoma, which were associated with the same anatomical locations . Epigenetic factors may be involved in the pathogenesis of brainstem schwannomas, and further research on epigenetics is required.
We experienced the first case of brainstem schwannoma recurrence after resection, which was successfully controlled by irradiation. To the best of our knowledge, this study is the first to report the results of a genetic examination of brainstem intraparenchymal schwannoma. Our genetic analysis revealed that the tumor did not have any somatic variants pertinent to schwannoma, such as in NF2 and 22q loss. This case study suggests that brainstem schwannomas may differ from conventional schwannomas in their genetic background.
Availability of data and materials
The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.
Epithelial membrane antigen
Glial fibrillary acidic protein
Magnetic resonance imaging
Bigner DD, McLendon RE, Bruner JM, Russell DS. Russell and Rubinstein’s pathology of tumors of the nervous system. 6th ed. Oxford University Press; 1998.
Lin J, Feng H, Li F, Zhao B, Guo Q. Intraparenchymal schwannoma of the medulla oblongata. Case report. J Neurosurg. 2003;98(3):621–4.
Ramos AA, Vega MA, Valencia HS, Garcia JC, Perez VC. Intraparenchymal schwannoma involving the brainstem in a young woman. Pediatr Neurol. 2013;48(6):472–4.
Sharma AK, Savardekar AR, Nandeesh BN, Arivazhagan A, Rao MB. Intrinsic brainstem schwannoma—a rare clinical entity and a histological enigma. J Neurosci Rural Pract. 2016;7(2):302–4.
Agnihotri S, Jalali S, Wilson MR, Danesh A, Li M, Klironomos G, et al. The genomic landscape of schwannoma. Nat Genet. 2016;48(11):1339–48.
Smith MJ, Wallace AJ, Bowers NL, Eaton H, Evans DG. SMARCB1 mutations in schwannomatosis and genotype correlations with rhabdoid tumors. Cancer Genet. 2014;207(9):373–8.
Rodriguez-Salazar A, Carrillo R, de Miguel J. Intracerebral schwannoma in a child: report of a case. Childs Brain. 1984;11(1):69–72.
Erongun U, Ozkal E, Acar O, Uygun A, Kocaogullar Y, Gungor S. Intracerebral schwannoma: case report and review. Neurosurg Rev. 1996;19(4):269–74.
Maiuri F, Colella G, D’Acunzi G, De Caro MB. Malignant intracerebellar schwannoma. J Neurooncol. 2004;66(1–2):191–5.
Prakash B, Roy S, Tandon PN. Schwannoma of the brain stem: case report. J Neurosurg. 1980;53(1):121–3.
Aryanpur J, Long DM. Schwannoma of the medulla oblongata. Case report. J Neurosurg. 1988;69(3):446–9.
Ladouceur D, Bergeron D, Lamarche JB, Lamontagne L. Cystic schwannoma of the brainstem. Can J Neurol Sci. 1989;16(3):357–60.
Sharma V, Newton G. Schwannoma of the medulla oblongata. Br J Neurosurg. 1993;7(4):427–9.
Sharma MC, Karak AK, Gaikwad SB, Mahapatra AK, Mehta VS, Sudha K. Intracranial intraparenchymal schwannomas: a series of eight cases. J Neurol Neurosurg Psychiatry. 1996;60(2):200–3.
Tanabe M, Miyata H, Okamoto H, Watanabe T, Hori T, Masakawa A, et al. Brainstem schwannoma—case report. Neurol Med Chir. 1996;36(12):880–3.
Muzzafar S, Ketonen L, Weinberg JS, Schellingerhout D. Imaging and clinical features of an intra-axial brain stem schwannoma. AJNR Am J Neuroradiol. 2010;31(3):567–9.
Srivastav AK, Tungaria A, Kumar R, Sahu RN. Intraparenchymal schwannoma of brainstem in a pediatric patient. Neurol India. 2011;59(6):924–6.
Gao Y, Qin Z, Li D, Yu W, Sun L, Liu N, et al. Intracerebral schwannoma: a case report and literature review. Oncol Lett. 2018;16(2):2501–10.
Lee SH, Yoo H, Lee JH, Cho KJ, Rhee CH, Jang JS, et al. Multiple intraparenchymal schwannomas in the cerebellum, brainstem, and cervical spinal cord. Acta Neurochir. 1999;141(7):779–80.
Nelson E, Rennels M. Innervation of intracranial arteries. Brain. 1970;93(3):475–90.
Feigin I, Ogata J. Schwann cells and peripheral myelin within human central nervous tissues: the mesenchymal character of Schwann cells. J Neuropathol Exp Neurol. 1971;30(4):603–12.
Scherer SS. The biology and pathobiology of Schwann cells. Curr Opin Neurol. 1997;10(5):386–97.
Mirsky R, Woodhoo A, Parkinson DB, Arthur-Farraj P, Bhaskaran A, Jessen KR. Novel signals controlling embryonic Schwann cell development, myelination and dedifferentiation. J Peripher Nerv Syst. 2008;13(2):122–35.
Woodhoo A, Sommer L. Development of the Schwann cell lineage: from the neural crest to the myelinated nerve. Glia. 2008;56(14):1481–90.
Furlan A, Adameyko I. Schwann cell precursor: a neural crest cell in disguise? Dev Biol. 2018;444(Suppl 1):S25–35.
Jessen KR, Mirsky R. Schwann cell precursors; multipotent glial cells in embryonic nerves. Front Mol Neurosci. 2019;12:69.
Bian LG, Tirakotai W, Sun QF, Zhao WG, Shen JK, Luo QZ. Molecular genetics alterations and tumor behavior of sporadic vestibular schwannoma from the People’s Republic of China. J Neurooncol. 2005;73(3):253–60.
Chen H, Xue L, Wang H, Wang Z, Wu H. Differential NF2 gene status in sporadic vestibular schwannomas and its prognostic impact on tumour growth patterns. Sci Rep. 2017;7(1):5470.
Hadfield KD, Smith MJ, Urquhart JE, Wallace AJ, Bowers NL, King AT, et al. Rates of loss of heterozygosity and mitotic recombination in NF2 schwannomas, sporadic vestibular schwannomas and schwannomatosis schwannomas. Oncogene. 2010;29(47):6216–21.
Havik AL, Bruland O, Myrseth E, Miletic H, Aarhus M, Knappskog PM, et al. Genetic landscape of sporadic vestibular schwannoma. J Neurosurg. 2018;128(3):911–22.
Lassaletta L, Torres-Martin M, Pena-Granero C, Roda JM, Santa-Cruz-Ruiz S, Castresana JS, et al. NF2 genetic alterations in sporadic vestibular schwannomas: clinical implications. Otol Neurotol. 2013;34(7):1355–61.
Kullar PJ, Pearson DM, Malley DS, Collins VP, Ichimura K. CpG island hypermethylation of the neurofibromatosis type 2 (NF2) gene is rare in sporadic vestibular schwannomas. Neuropathol Appl Neurobiol. 2010;36(6):505–14.
Lee JD, Kwon TJ, Kim UK, Lee WS. Genetic and epigenetic alterations of the NF2 gene in sporadic vestibular schwannomas. PLoS ONE. 2012;7(1):e30418.
We are grateful to the patient and his father for permitting the use of clinical and genetic data.
This study was supported by grants to Dr. Nobuhito Saito from the Japan Society for the Promotion of Science (Scientific Research [B], No. 17H04301) and to Dr. Satoru Miyawaki from the Japan Society for the Promotion of Science (Scientific Research [C], No. 19K09473) and Takeda Science Foundation.
Ethics approval and consent to participate
This study was approved by the Human Genome, Gene Analysis Research Ethics Committee of the Faculty of Medicine, the University of Tokyo (Approval Number G10028). Written informed consent was obtained from the patient and his father for the publication of the present case report.
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Ishigami, D., Miyawaki, S., Nakatomi, H. et al. Brainstem intraparenchymal schwannoma with genetic analysis: a case report and literature review. BMC Med Genomics 14, 205 (2021). https://doi.org/10.1186/s12920-021-01049-z
- Intraparenchymal schwannoma
- Case report