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Novel compound heterozygous mutations in the desert hedgehog (DHH) gene in cases of siblings with 46,XY disorders of sexual development

Abstract

Background

Disorders of sex development (DSD) are congenital disorders in which the development of the chromosomal, gonadal, or anatomical sex is atypical. Mutations in various genes can impede gonadal development, hormone synthesis, or hormone function and cause DSD.

Methods

Exome sequencing was performed for two siblings with 46,XY DSD. All mutations identified by exome sequencing were confirmed by Sanger sequencing.

Results

The 13-month-old younger sibling had a female appearance of the external genital with a clitoris that was assessed as Prader III and scored 2 in the external masculinization score evaluative test. The 16-year-old elder sibling had severe hypospadias. Exome sequencing revealed compound heterozygous mutations in exon 3 of DHH in the siblings with 46,XY DSD. The frameshift mutation (NM_021044.3: c.602delC) was derived from the father and was predicted to be deleterious. The (c.937G > T) substitution mutation was derived from the mother.

Conclusions

Novel compound heterozygous mutations of DHH led to 46,XY DSD in two siblings. This study expands the phenotypic mutation spectra of DHH in patients with 46,XY DSD.

Peer Review reports

Background

Disorders of sex development (DSD) are congenital disorders in which the development of the chromosomal, gonadal, or anatomical sex is atypical. DSD includes a wide range of phenotypes, such as 46,XY DSD, 46,XX DSD, and chromosomal DSD [1]. The incidence rate among subjects with 46,XY to have a DSD has been estimated to be 1 in 20,000 births globally [2]. For the individuals with 46,XY DSD, defects in the development of the male gonads and/or external genitalia occur.

Male sex determination in humans depends on the SRY gene, which is located on the Y chromosome. It also depends on the presence of several other genes located on autosomal and X-linked loci that are involved in the testis-determining pathways [3], including SOX9, MAP3K1, NR5A1, WT1, ARTX, and DHH.

DHH, encoding desert hedgehog (DHH), belongs to the hedgehog gene family. This gene encodes signalling molecules that play an essential role in cell proliferation, differentiation, tissue patterning, and regulation morphogenesis [4]. DHH is located on 12q12-q13.1. It is composed of three exons and encodes a protein of 396 amino acid residues [5]. DHH mRNA and protein are explicitly expressed in Sertoli cells and Schwann cells along peripheral nerves [6]. It is one of the first indications of male-specific development before overt sexual dimorphism of the gonads. Moreover, it promotes foetal Leydig cell development and the consequent formation of testis cords by activating Hedgehog signalling [7]. In humans, mutations in DHH have been reported in patients presenting with 46,XY DSD and with/without minifascicular polyneuropathy [5, 8,9,10]. Interestingly, previous literature has reported that these patients always have homozygous DHH mutations or compound heterozygote mutations [5, 9, 11, 12].

This study reports the genetic and molecular analysis of two siblings with 46,XY DSD who had a novel double heterozygous variant in DHH.

Methods

Study participants

This study was reviewed and approved by the Institute’s Human Research Committee of the Children’s Hospital of Zhejiang University School of Medicine. The patients were evaluated by a multidisciplinary team in the hospital, including paediatric urologists, paediatric endocrinologists, and geneticists. The parents of the siblings approved the usage of blood samples for the study.

Genetic analysis

Genomic DNA from the proband was extracted using a QIAamp Blood Midi Kit (QIAGEN, Valencia, CA). To identify disease-causing gene variants, a GenCap panel with 168 genes associated with DSD was customized, and a capture strategy was performed using the GenCap custom enrichment kit (MyGenostics Inc, Beijing, China). An Illumina HiSeq 4000 sequencer (Illumina, San Diego, CA, USA) was used with 150 bp paired-end reads.

Data analysis

Following sequencing, the raw data were saved as a FASTQ format. Both MGI sequencing adapters and low quality reads (< 80 bp) were filtered by cutadaptor software (http://code.google.com/p/cutadapt/). The clean reads were mapped to the UCSC hg19 human reference genome using the parameter BWA of Sentieon software.(https://www.sentieon.com/).The duplicated reads were removed using the parameter driver of Sentieon software, and the parameter driver is used to correct the base, so that the quality value of the base in the reads of the final output BAM file can be closer to the real probability of mismatch with the reference genome, and the mapped reads were used for the detection of variation. The variants of SNP and InDel were detected by the parameter driver of Sentieon software. Then, the data would be transformed to VCF format. Variants were further annotated by ANNOVAR software (http://annovar.openbioinformatics.org/en/latest/), and associated with multiple databases, such as, 1000 genome, ESP6500, dbSNP, EXAC, Inhouse (MyGenostics), HGMD, and also predicted by SIFT, PolyPhen-2, MutationTaster, GERP +  + . The pathogenicity of variation loci was also analyzed according to ACMG (American College of Medical Genetics and Genomics) genetic variation classification criteria and guidelines.

Validation by sanger sequencing

All mutations identified by HiSeq 4000 sequencing were confirmed by Sanger sequencing. Genomic DNA from all available family members were obtained for Sanger sequencing. A pair of primers were designed to amplify the exon 3 of DHH(NM_021044.3). Exon 3-F: 5’- TAGCGCGTGCAGCAGTCT-3’; exon 3-R: 5’- TGGTCTTGATTCAATCCTCCC-3’.

The PCR amplifications were performed as per the following procedure: an initial denaturation of 98 °C for 2 min, 10 cycles of denaturation at 98 °C for 10 s, annealing at 65 °C for 30 s, extension at 72 °C for 10 se c, then 25 cycles of denaturation at 98 °C for 10 s, annealing at 55 °C for 30 s, extension at 72 °C for 10 s and a final extension of 72 °C for 1 min. An ABI3730xl sequencer was used for sanger sequencing of the purified PCR products. The sequencing results were analyzed using the DNASTAR(Madison) software.

Results

Clinical manifestations

The two siblings were born at full term in Zhejiang Province, China. The pregnancies and perinatal periods of both patients were not uneventful. They had average height and weight for their age, according to the Chinese children’s standards. No significant disorders were found in the parents. The patients were evaluated by the DSD multidisciplinary team in the hospital, including paediatric urologists, paediatric endocrinologists, and geneticists.

Patient 1

The proband was the younger brother; he was 13-month-old. His social sex was female. His mother delivered him at 38 years old. He had a single perineal urogenital orifice and a mega-clitoris with almost complete scrotum fusion. His bilateral testes were palpable in the inguinal regions. He was assessed as stage III on the Prader scale and scored 5 on an external masculinization score (EMS) evaluative test (Fig. 1A and B). The karyotype was determined to be 46,XY, and the SRY gene was positive. The serum dihydrotestosterone level was lower than the normal range (17.48 pg/ml). The serum inhibin B concentration was 314.63 pg/ml. The 17-α hydroxyprogesterone concentration was 0.3 nmol/L. The AMH concentration was 103.37 ng/ml. The serum levels of FSH, LH, oestradiol, progesterone, prolactin, testosterone, HCG, adrenocorticotropin, and hydrocortisone were normal. The ultrasound test showed that two testes were in the inguinal regions. The volume of the left testis was 1.3 × 0.8 × 0.6 cm, and the volume of the right testis was 1.2 × 0.8 × 0.7 cm. His diagnosis was 46,XY DSD.

Fig. 1
figure 1

External and internal genitalia features of proband patient 1. (A and B) External genitalia appeared as a single perineal urogenital orifice and a mega-clitoris with almost complete scrotum fusion. (C) The left vas deferens (arrow). (D) The right vas deferens (arrow). (E) The left testis was in the left inguinal region. (F) The right testis was in the right inguinal region

The patient underwent laparoscopic and cystoscopy explorations. The spermatic vessels and the deferent ducts were identified (Fig. 1C and D). The verumontanum was found. The investigation did not find a uterus, ovary, or vagina. The volumes of the bilateral testes were significantly smaller than average (Fig. 1E and F). However, the appearance of the testes and epididymis seemed normal. Bilateral gonadal biopsy was performed, and the pathological diagnosis confirmed that the bilateral gonads were testes. Finally, he underwent bilateral orchidopexy and two-stage hypospadias repair. The surgeries were performed successfully. During the 1-year follow-up, the appearance of the external genitalia was similar to that of normal males of his age; however, his penis was significantly shorter than that of normal males. Even so, the parents were extremely satisfied with the outcomes of the treatments.

Patient 2

The elder brother was a 16-year-old male who was also diagnosed with 46,XY DSD. His mother delivered him at 23 years old. He had had a single perineal urogenital orifice and a mega-clitoris with complete scrotum fusion. His bilateral testes were in the scrotums. He had been assessed as stage III on the Prader scale and scored 6 on the EMS evaluative test according to his medical history record. He had received the two-stage hypospadias repair surgeries in his childhood (aged 3 years old) in our hospital. Currently, the penis is smaller than the average range according to his age, and the urethral orifice is opened in the top of the glans. Two testes were palpable in the scrotums. The volumes of the testes were normal. The breasts were mildly developed as stage 2. The boy refused our photograph requests. His karyotype was identified as 46,XY, and the SRY gene was positive. The nerve conduction study results were normal. The ultrasound showed that the length of the penis was 7.6 cm. The volume of the left testicle was 3.9 × 2.0 × 1.9 cm, and the volume of the right testicle was 4.0 × 2.2 × 2.0 cm. The volume of the left breast was 1.8 × 1.4 × 0.7 cm, and the volume of the right breast was 2.0 × 1.9 × 0.6 cm.

Genetic sequencing analysis

Next-generation sequencing was used to analyse the genes of the proband. The SRY gene was analysed in the two patients, and no mutations were found. Direct sequencing of DHH indicated a double heterozygous mutation in exon 3 (DHH; NM_021044.3) in both brothers. The c.602delC mutation was derived from their father, and the c.937G > T mutation was derived from their mother. The c.602delC mutation was a frameshift mutation that subsequently caused 136 amino acid changes. The c.937G > T mutation was a missense mutation that led to the change of a valine to a leucine at position 313 of the protein, according to the reference sequence NM_021044. The incidence is 1/16 for the siblings to share the same DHH mutation. No sequence variations were found in exon 1 or exon 2 of the gene. The next-generation sequencing results were verified by Sanger sequencing (Fig. 2A) using a pair of primers designed to amplify exon 3 of DHH.

Fig. 2
figure 2

Genetic analysis of two siblings with 46,XY DSD. (A) Pedigree of the family. Squares indicate males, and circles indicate females. Dashed squares symbolize females or males affected with 46,XY DSD. The single line connects the two parents, who are not in a consanguineous marriage. The proband (II/1) was a 13-month-old male. His elder brother (II/2) is currently 16 years old but was 3 years old at the time of diagnosis. (B) Validation of the variants by Sanger sequencing of the parents and two patients

All pathogenicity prediction algorithms (SIFT, PolyPhen2, and CADD) consider this mutation damaging or disease-causing. This variant is not present in the 1000 Genomes Project, dbSNP, or ExAC databases. Furthermore, we did not find any other mutation in DSD-related genes.

The pedigree of the family is shown in Fig. 2B.

Discussion

DHH belongs to the hedgehog family, which consists of sonic hedgehog (SHH), DHH, and India hedgehog (IHH). They function as cell-to-cell signals and have embryonic roles in the specification of tissue patterning and cell differentiation [13]. The importance of DHH in male sexual development has been indicated in various studies. It has been reported that a homozygous mutation in the DHH gene can result in complete pure gonadal dysgenesis [10], partial gonadal dysgenesis [8], and even gonadal tumours [9] in patients with 46,XY. However, DHH gene mutations causing 46,XY have rarely been reported in the Chinese population [14, 15]. In a putative animal model study, DHH regulates testis formation, spermatogenesis, and the differentiation of peritubular myoid cells [16]. A missense mutation of the gene encoding DHH in mutant rats indicated that the Dhh gene might be essential for developing Leydig cells [17] and functions to secrete testosterone through a paracrine signalling mechanism. Furthermore, DHH is involved in the WT1-SOX8/SOX9-beta-CATENIN-DHH network in Sertoli cells, which is the signalling pathway for testis cord maintenance [18].

The DHH signalling pathway may ensure mammalian spermatogenic function and may be directly regulated by sex-determining pathways. DHH can be autocatalytically cleaved into the N-terminal portion and C-terminal portion. The N-terminal portion is soluble and contains the signalling activity [19]. The Hedgehog receptor Patched negatively regulates the activity and ciliary accumulation of Smoothened, a seven-transmembrane protein that is essential for transducing Hedgehog signalling [20]. DHH signalling is mediated by hedgehog coreceptors and cell adhesion molecules, and is downregulated by oncogenes (CDO and BOC). CDO and BOC bind all mammalian Hedgehog proteins in a conserved manner [9, 13]. Previous research has shown that BOC positively regulates hedgehog signalling [21]. The C-terminal portion is involved in precursor processing, which covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. In this study, the DHH frameshift mutation (c.602delC) resulted in changes to 136 amino acids, and the missense mutation (c.937G > T) led to the replacement of V with L at residue 313. These mutations may alter the normal functions of DHH by either restricting the processing or secretion of the N-terminal portion or by limiting its binding to its receptor Patched or coreceptors such as CDO and BOC. Thus, these changes would lead to incomplete sex development.

As we showed before, patient 1 was a 13-month-old male who was of female social sex, and patient 2 was a 16-year-old male. However, they had the same DHH gene mutations. The external genitalia of patient 1 were feminized, and two gonads were linguistic. In contrast, the external genitalia of patient 2 were penoscrotal hypospadias. The severity of DSD in these two patients with the same gene mutations indicates that male sex development may be associated with gene mutations and affected by penetrance of the gene, which may be due to pregnancy age or the mother’s living environment. The mother of both patients gave birth the younger brother when she was 38 years old, and the family moved from the rural area to the city.

Genetic analysis is a useful tool for doctors to determine the causes of diseases, make a specific diagnosis, and precisely treat an individual patient. In previous studies, most of the patients were diagnosed in adolescence or adulthood, and the chief complaint was amenorrhea, which meant that their social sex was female [22]. There has been no evidence to indicate their brain sex or the subsequent treatment after DSD diagnosis in these studies. However, an early diagnosis appears to be beneficial to the patient and family. Parents and paediatric urologists will have more time to discuss any issues and make better treatment choices to minimize harm to the children physically and psychologically.

Conclusions

We identified novel compound heterozygous DHH mutations (c.602delC and c.937G > T) in two siblings diagnosed with 46,XY DSD. The c.602delC mutation caused changes to the subsequent 136 amino acids, and the c.937G > T mutation was a missense mutation that changed a valine residue to a leucine residue at position 313 of the protein. These two mutations suppress the normal functions of DHH, which affects the hedgehog signalling pathway.

Availability of data and materials

The raw sequence data reported in this paper have been deposited in NCBI-SRA with the BioProject accession number PRJNA855469.

Abbreviations

DSD:

Disorders of sex development

BOC:

Brother of the cell adhesion molecule downregulated by oncogenes

SRY:

Sex-determining region Y

SOX9:

Sex-determining region Y-related high-mobility group box 9

MAP3K1:

Mitogen-activated protein kinase kinase kinase 1

NR5A1:

Nuclear receptor subfamily 5 group A member 1

WT1:

Wilms' tumour gene 1

EMS:

External masculinization score

AMH:

Anti-mullerian hormone

FSH:

Follicle-stimulating hormone

LH:

Luteinizing Hormone

HCG:

Human chorionic gonadotropin

SHH:

Sonic hedgehog

IHH:

India hedgehog

CDO:

Cell adhesion molecule downregulated by oncogenes

References

  1. Bakula DM, Sharkey CM, Wolfe-Christensen C, Mullins AJ, Meyer J, Mullins LL, Wisniewski AB. Recommendations for the Establishment of disorders/differences of sex development Interdisciplinary care clinics for youth. J Pediatr Nurs. 2017;37:79–85.

    Article  Google Scholar 

  2. Lee PA, Nordenstrom A, Houk CP, Ahmed SF, Auchus R, Baratz A, Baratz Dalke K, Liao LM, Lin-Su K, Looijenga LH 3rd, et al. Global disorders of sex development update since 2006: perceptions. Approach Care Horm Res Paediatr. 2016;85(3):158–80.

    CAS  Article  Google Scholar 

  3. Canto P, Vilchis F, Soderlund D, Reyes E, Mendez JP. A heterozygous mutation in the desert hedgehog gene in patients with mixed gonadal dysgenesis. Mol Hum Reprod. 2005;11(11):833–6.

    CAS  Article  Google Scholar 

  4. Choy SW, Cheng SH. Hedgehog signaling. Vitam Horm. 2012;88:1–23.

    CAS  Article  Google Scholar 

  5. Paris F, Flatters D, Caburet S, Legois B, Servant N, Lefebvre H, Sultan C, Veitia RA. A novel variant of DHH in a familial case of 46, XY disorder of sex development: Insights from molecular dynamics simulations. Clin Endocrinol (Oxf). 2017;87(5):539–44.

    CAS  Article  Google Scholar 

  6. Parmantier E, Lynn B, Lawson D, Turmaine M, Namini SS, Chakrabarti L, McMahon AP, Jessen KR, Mirsky R. Schwann cell-derived desert hedgehog controls the development of peripheral nerve sheaths. Neuron. 1999;23(4):713–24.

    CAS  Article  Google Scholar 

  7. Chen H, Wang Y, Ge R, Zirkin BR. Leydig cell stem cells: Identification, proliferation and differentiation. Mol Cell Endocrinol. 2017;445:65–73.

    CAS  Article  Google Scholar 

  8. Rothacker KM, Ayers KL, Tang D, Joshi K, van den Bergen JA, Robevska G, Samnakay N, Nagarajan L, Francis K, Sinclair AH, et al. A novel, homozygous mutation in desert hedgehog (DHH) in a 46, XY patient with dysgenetic testes presenting with primary amenorrhoea: a case report. Int J Pediatr Endocrinol. 2018;2018:2.

    Article  Google Scholar 

  9. Werner R, Merz H, Birnbaum W, Marshall L, Schroder T, Reiz B, Kavran JM, Baumer T, Capetian P, Hiort O. 46,XY gonadal dysgenesis due to a homozygous mutation in desert hedgehog (DHH) identified by exome sequencing. J Clin Endocrinol Metab. 2015;100(7):E1022–9.

    CAS  Article  Google Scholar 

  10. Canto P, Soderlund D, Reyes E, Mendez JP. Mutations in the desert hedgehog (DHH) gene in patients with 46, XY complete pure gonadal dysgenesis. J Clin Endocrinol Metab. 2004;89(9):4480–3.

    CAS  Article  Google Scholar 

  11. Sato NS, Maekawa R, Ishiura H, Mitsui J, Naruse H, Tokushige SI, Sugie K, Tate G, Shimizu J, Goto J, et al. Partial duplication of DHH causes minifascicular neuropathy: a novel mutation detection of DHH. Ann Clin Transl Neurol. 2017;4(6):415–21.

    CAS  Article  Google Scholar 

  12. Callier P, Calvel P, Matevossian A, Makrythanasis P, Bernard P, Kurosaka H, Vannier A, Thauvin-Robinet C, Borel C, Mazaud-Guittot S, et al. Loss of function mutation in the palmitoyl-transferase HHAT leads to syndromic 46, XY disorder of sex development by impeding hedgehog protein palmitoylation and signaling. PLoS Genet. 2014;10(5): e1004340.

    Article  Google Scholar 

  13. Kavran JM, Ward MD, Oladosu OO, Mulepati S, Leahy DJ. All mammalian Hedgehog proteins interact with cell adhesion molecule, down-regulated by oncogenes (CDO) and brother of CDO (BOC) in a conserved manner. J Biol Chem. 2010;285(32):24584–90.

    CAS  Article  Google Scholar 

  14. Xu Y, Wang Y, Li N, Yao R, Li G, Li J, Ding Y, Chen Y, Huang X, Chen Y, et al. New insights from unbiased panel and whole-exome sequencing in a large Chinese cohort with disorders of sex development. Eur J Endocrinol. 2019;181(3):311–23.

    CAS  Article  Google Scholar 

  15. Qin XY, Dong WK, Wang W, Dong ZY, Xiao Y, Lu WL, Wang DF. Analysis of clinical features and related genes variation in five patients with 46, XX male syndrome. Zhonghua Er Ke Za Zhi. 2016;54(11):840–3.

    CAS  PubMed  Google Scholar 

  16. Bitgood MJ, Shen L, McMahon AP. Sertoli cell signaling by desert hedgehog regulates the male germline. Curr Biol. 1996;6(3):298–304.

    CAS  Article  Google Scholar 

  17. Kawai Y, Noguchi J, Akiyama K, Takeno Y, Fujiwara Y, Kajita S, Tsuji T, Kikuchi K, Kaneko H, Kunieda T. A missense mutation of the Dhh gene is associated with male pseudohermaphroditic rats showing impaired Leydig cell development. Reproduction. 2011;141(2):217–25.

    CAS  Article  Google Scholar 

  18. Chen SR, Liu YX. Testis cord maintenance in mouse embryos: genes and signaling. Biol Reprod. 2016;94(2):42.

    PubMed  Google Scholar 

  19. Uemura Y, Nakagawa N, Wakamatsu T, Kim K, Montelione GT, Hunt JF, Kuramitsu S, Masui R. Crystal structure of the ligand-binding form of nanoRNase from bacteroides fragilis, a member of the DHH/DHHA1 phosphoesterase family of proteins. FEBS Lett. 2013;587(16):2669–74.

    CAS  Article  Google Scholar 

  20. Kim J, Hsia EY, Brigui A, Plessis A, Beachy PA, Zheng X. The role of ciliary trafficking in hedgehog receptor signaling. Sci Signal. 2015;8(379):ra55.

    Article  Google Scholar 

  21. Tenzen T, Allen BL, Cole F, Kang JS, Krauss RS, McMahon AP. The cell surface membrane proteins Cdo and Boc are components and targets of the hedgehog signaling pathway and feedback network in mice. Dev Cell. 2006;10(5):647–56.

    CAS  Article  Google Scholar 

  22. Domenice S, Arnhold IJP, Costa EMF, Mendonca BB. 46,XY Disorders of Sexual Development. In: De Groot LJ, Chrousos G, Dungan K, Feingold KR, Grossman A, Hershman JM, Koch C, Korbonits M, McLachlan R, New M et al., editors. Endotext. South Dartmouth (MA); 2000.

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Acknowledgements

We are thankful to the family for their contribution to this study.

Funding

This work was supported by the Zhejiang Provincial Natural Science Foundation of China (LY19H040012) and the National Key Research and Development Project (2018YFC1002700). The funding bodies played no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.

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Authors

Contributions

JW and JQW conducted the experiments and have drafted the work. GC interpretation of data. JW, WR and LG acquisition, analysis, and interpretation of data. DT made substantial contributions to the conception and design of the work. were responsible for the data collection and interpretation and literature review and wrote the manuscript. All authors contributed to the article and approved the submitted version. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Daxing Tang.

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Ethics approval and consent to participate

The study was approved by the Ethics Committee of Children’s Hospital, Zhejiang University School of Medicine. Written informed consent to participate was obtained from all of the participants aged 16 and over. For patients younger than 16 years of age, written informed consent was obtained from the parents/guardians of the patient. A copy of the written consent is available for review by the editor of this journal.

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Written informed consent was obtained from the patient or parents/guardians of the patient for publication of clinical details and/or clinical images.

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The authors declare that they have no competing interests.

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Wei, J., Wu, J., Ru, W. et al. Novel compound heterozygous mutations in the desert hedgehog (DHH) gene in cases of siblings with 46,XY disorders of sexual development. BMC Med Genomics 15, 178 (2022). https://doi.org/10.1186/s12920-022-01334-5

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Keywords

  • Disorders of sex development
  • Desert hedgehog (DHH)
  • Mutation