- Research article
- Open Access
- Open Peer Review
Saliva samples are a viable alternative to blood samples as a source of DNA for high throughput genotyping
- Jean E Abraham†1, 2, 3, 4Email author,
- Mel J Maranian†1,
- Inmaculada Spiteri3,
- Roslin Russell3,
- Susan Ingle2, 4,
- Craig Luccarini1,
- Helena M Earl2, 4,
- Paul PD Pharoah1,
- Alison M Dunning1 and
- Carlos Caldas1, 2, 3, 4
© Abraham et al.; licensee BioMed Central Ltd. 2012
- Received: 28 November 2011
- Accepted: 3 May 2012
- Published: 30 May 2012
The increasing trend for incorporation of biological sample collection within clinical trials requires sample collection procedures which are convenient and acceptable for both patients and clinicians. This study investigated the feasibility of using saliva-extracted DNA in comparison to blood-derived DNA, across two genotyping platforms: Applied Biosystems TaqmanTM and Illumina BeadchipTM genome-wide arrays.
Patients were recruited from the Pharmacogenetics of Breast Cancer Chemotherapy (PGSNPS) study. Paired blood and saliva samples were collected from 79 study participants. The Oragene DNA Self-Collection kit (DNAgenotek®) was used to collect and extract DNA from saliva. DNA from EDTA blood samples (median volume 8 ml) was extracted by Gen-Probe, Livingstone, UK. DNA yields, standard measures of DNA quality, genotype call rates and genotype concordance between paired, duplicated samples were assessed.
Total DNA yields were lower from saliva (mean 24 μg, range 0.2–52 μg) than from blood (mean 210 μg, range 58–577 μg) and a 2-fold difference remained after adjusting for the volume of biological material collected. Protein contamination and DNA fragmentation measures were greater in saliva DNA. 78/79 saliva samples yielded sufficient DNA for use on Illumina Beadchip arrays and using Taqman assays. Four samples were randomly selected for genotyping in duplicate on the Illumina Beadchip arrays. All samples were genotyped using Taqman assays. DNA quality, as assessed by genotype call rates and genotype concordance between matched pairs of DNA was high (>97%) for each measure in both blood and saliva-derived DNA.
We conclude that DNA from saliva and blood samples is comparable when genotyping using either Taqman assays or genome-wide chip arrays. Saliva sampling has the potential to increase participant recruitment within clinical trials, as well as reducing the resources and organisation required for multicentre sample collection.
- Saliva Collection
- Microchip Electrophoresis
- Breast Cancer Chemotherapy
- Genotype Concordance
- Paired Blood
Incorporation of biological sample collection within clinical trials and cohort studies will enhance our ability to identify biomarkers that improve individualisation of treatments. This requires sample collection procedures which are convenient and acceptable for both patients and clinicians. Whilst blood sampling and tumour biopsies are essential in some circumstances, often a less invasive procedure is sufficient and would improve trial recruitment. The potential advantages of saliva sample collection compared with blood sample collection include lower overall cost, lower infection risk, increased patient convenience, acceptability, compliance and uptake. However potential disadvantages include lower mean DNA yield and greater contamination with bacterial DNA. This study investigates the suitability of DNA extracted from saliva compared with DNA extracted from blood for high-throughput genotyping platforms.
Buccal sampling as an alternative to venous blood sampling has been investigated previously, but there is still reluctance to using DNA extracted from saliva samples or buccal cyto-brushes [1–7]. The main reasons for this are concerns over reduced yield and quality of DNA extracted from saliva, especially given the rigorous DNA requirements for high-throughput technologies. Studies investigating bacterial content in saliva collection samples have estimated that the median bacterial content from Oragene saliva collection kits is 11.8%, whereas bacterial content from mouthwash and buccal swabs was up to 60% and 90% respectively . One study (n = 23), using the Illumina HumanHAP300 beadchip, concluded that under optimal conditions DNA from buccal cells provided comparable results to blood-derived DNA . Another study successfully used saliva-extracted DNA in a Genome Wide Association Study (GWAS), but there was no clear comparison of the mean DNA yield or range of DNA yield found with each sample type. However the study did show comparable concordance and call rates . One published study in dogs compared the performance of paired canine saliva and blood-derived DNA using the Illumina Infinium platform. This study demonstrated that canine saliva DNA was suitable for high-throughput genotyping studies .
This study compares DNA extracted from blood and saliva across two genotyping platforms. The Applied Biosystems TaqmanTM platform allowed comparison of paired saliva and blood DNA samples in 79 study participants, while the Illumina beadchipsTM compared genotyping quality across thousands of SNPs in four participants.
The study also compared: (i) DNA yields from normal extraction procedures on 9 ml EDTA blood tubes (monovette/vacutainer) and Oragene DNA Saliva Self-Collection kits (DNA Genotek®) (ii) Ratios of Absorbance at 260 and 280 nm (iii) DNA fragmentation (iv) Genotype Call Rates (i.e. the number of results obtained) for each type of DNA and (v) Genotype Concordances between matched pairs of samples – a measure of the accuracy of the genotypes obtained.
Paired blood and saliva samples (n = 79) were collected from a subset of patients participating in the Pharmacogenetics of Breast Cancer Chemotherapy (PGSNPS) study, which recruited patients from four UK breast cancer chemotherapy studies [12–14]. After obtaining written, informed consent, two EDTA blood samples (median volume 8 ml) were initially collected from all participants.
Subsequently participants were randomly selected for the saliva-blood feasibility study. These participants were contacted by mail and received an information leaflet explaining the aims and requirements of the study, a consent form which the patient signed, if they wished to participate, or alternatively a form to decline entry into the study and a stamped addressed envelope. If the patient consented, a trial research nurse or doctor would co-sign the consent form, retain a copy centrally and return a copy to the patient, enclosing the Oragene DNA Self-Collection kit (DNA Genotek®) together with the manufacturer’s instructions. Eighty-five percent of patients approached regarding the saliva-blood feasibility study gave consent and entered the study. The self-collection method did not require the participation of a regional hospital to recruit the patient. Patients recruited to this feasibility study returned the samples by post or in person, over a period of 2 months from April 2008 to May 2008. Samples were received by a nominated research nurse who stored the samples at room temperature until the samples were brought to the laboratory for extraction. As samples were received at different time points, different samples will have been stored for variable lengths of time.
The saliva-blood feasibility study received ethical approval in April 2008 from the Cambridgeshire 1 (formerly Huntingdon) Research Ethics Committee, UK.
Saliva DNA extraction
The Oragene saliva collection device was selected for use because at the time the study was initiated this was the most well-established and commonly used saliva collection system. DNA was extracted from a 500 μl aliquot from the Oragene DNA/saliva Self-Collection kits in accordance with the manufacturer’s instructions . Each sample was bar-coded, and stored until required for DNA extraction. Extracted samples were stored at −20°C prior to genotyping. DNA was quantified using Picogreen (Invitrogen, Ltd), according to manufacturer’s instructions, which specifically measures double stranded DNA, thus ensuring more accurate measurement of the human DNA component of total saliva DNA. All samples were normalised to 2 ng/μl, based on the Picogreen quantification, prior to genotyping.
Blood DNA extraction
Lymphocyte DNA has been extracted and normalised to 40 ng/μl for storage by Gen-Probe, Livingstone, UK, using a single-tube chloroform extraction procedure. Subsequently this DNA was normalised to 2 ng/μl for genotyping
Genotyping was performed using Taqman® according to manufacturer’s instructions. Primers and probes (FAM and VIC labelled) were supplied directly by Applied Biosystems as Assays-by-DesignTM (see Additional file 1: Figure S1). Saliva DNA samples were plated out in duplicate on a 384 well plate, alongside additional duplicate DNAs taken from the paired blood samples (all normalised to 2 ng/μl). Plates were read on the ABI Prism 7900 using the Sequence Detection Software (Applied Biosystems). Four assays, which had been previously used in other research projects (data not shown) and therefore known to work well, were chosen for this experiment.
Genome wide genotyping of paired blood and saliva samples using HumanHap duo BeadChip arrays
DNA from four saliva samples was genotyped in duplicate using HumanHap300 Duo Illumina BeadChip arrays. DNA from matching blood samples was genotyped in duplicate using HumanHap550 Duo BeadChips. The change in array was due to the manufacturers stopping availability of HumanHap300 Duo Illumina BeadChip arrays, during the period the study was conducted. The assay was performed following manufacturer’s instructions  using 750 ng of each DNA sample, normalized to 50 ng/μl.
Spectrophotometry to assess DNA purity
Ten matched saliva (of varying yields) and blood samples were assessed for A260/A280 ratio (Additional file 1: Table S1). A 1/100 dilution was prepared (DNA/deionised H20) and analysed, using a CECIL CE2041 (2000 SERIES) spectrophotometer, according to the manufacturers instructions.
Microchip electrophoresis to assess fragmentation of DNA
Three matched saliva and blood DNA samples were randomly selected and assessed (see Additional file 1: Figure S2) using the Shimadzu Microchip Electrophoresis System and SYBR®Gold nucleic acid gel stain, (2 μl DNA in 4 μl DNA-500 Marker) according to manufacturer’s instructions.
DNA yields from blood and saliva
Sample volume collected (range)/ml
Mean DNA yield (range)/μg
Mean yield per ml sample collected (range)/μg
24 (0.2 - 52)
12 (0.1 - 26)
The quality of the DNA obtained was assessed by several criteria. Ratios of absorbance at 260 nm and 280 nm are presented in Additional file 1: Table S1. In this study DNA from blood has a mean (A260/A280 ratio) value (1.71), very close to the ideal value of 1.8. The mean (A260/A280 ratio) values from saliva is a little lower (1.56), indicating more protein contamination in the saliva extracted DNA. DNA fragmentation was compared on a bio-analyser and the results are shown in Additional file 1: Figure S2. Both DNA types have a clear high molecular weight band, but a more intense band of fragmented DNA is visible in DNA extracted from saliva.
Taqman genotype call rates (GCR)
Sample call rate blood DNA
Sample call rate saliva DNA
Illumina genotype call rates (GCR)
Mean SNP calls blood
Mean SNP calls saliva DNA
Taqman genotype concordance
Matched blood DNA
Matched saliva DNA
Matched blood/saliva DNA
Illumina genotype concordance
Matched blood DNA
Matched saliva DNA
Matched blood/saliva DNA
During the course of this study, Illumina changed their chip format such that the saliva DNA samples were genotyped on HumanHap300 Duo BeadChips (total 318,511 SNPs) while the matched blood DNA samples were genotyped on HumanHap550 Duo BeadChips (total 555,352 SNPs). We thus restricted our analyses to the 318,075 SNP assays common to the two formats (Tables 3 and 5).
These results demonstrate that saliva DNA was of comparable quality to blood DNA. A valid concern, prior to this study, was that saliva may contain significant proportions of DNA from oral bacteria and/or food. If this had been the case, the true concentration of human DNA added to each assay would have been below the Picogreen calculated concentration and overall call rates would have been proportionately reduced. We observed negligible evidence of any such problem in our assays, although we did observe evidence of greater protein contamination and DNA fragmentation in the saliva DNA.
This study has some limitations. The change in Illumina chip format made our study design sub-optimal, however, the very high concordance rates we observe between saliva and blood DNA despite the change in format demonstrate that this issue, which was beyond our control, has not affected the conclusions of our study. The actual volume of saliva received was not recorded. This value would have provided an estimate of patient compliance with manufacturers’ instructions for using the saliva collection kit and producing the required amount of saliva in the sample. However, the aim of this article was to establish whether DNA of an acceptable quality and quantity could be obtained to allow high-throughput genotyping on different platforms, which we have successfully demonstrated.
The results of this feasibility study have already informed the collection of saliva samples to increase recruitment into Pharmacogenetics of Breast Cancer Chemotherapy (PGSNPS) study. A total of 444 saliva samples have been collected and 19% of all the DNA samples analysed from this study to date have been saliva derived.
Individual saliva sampling kits are more expensive than blood sampling kits however this cost has to be counter-balanced by the increased inconvenience to the patient and the cost of trained staff required to obtain blood samples. In addition commercial extraction of DNA from saliva is cheaper than from blood (Gen-Probe extraction costs for blood £8/sample and for saliva £5.10/sample).
Although DNA yield is approximately 2-fold lower from saliva than from blood and other measures indicate slightly lower quality DNA, the key measure of genotyping quality is comparable on both Taqman and Illumina genome-wide beadchip arrays platforms. Saliva collection is less invasive than blood collection and participants who followed printed instructions, without supervision, provided useful quantities of DNA. The collection of saliva-derived DNA could substantially improve recruitment to translational studies in clinical trials, reduce costs and logistical problems associated blood collection within multicentre studies.
We would like to thank: the women participating in this study and the PGSNPS study team.
- Freeman B, Powell J, Ball D, Hill L, Craig I, Plomin R: DNA by mail: an inexpensive and noninvasive method for collecting DNA samples from widely dispersed populations. Behav Genet. 1997, 27 (3): 251-7. 10.1023/A:1025614231190.View ArticlePubMedGoogle Scholar
- Hayney MS, Dimanlig P, Lipsky JJ, Poland GA: Utility of a "swish and spit" technique for the collection of buccal cells for TAP haplotype determination. Mayo Clin Proc. 1995, 70 (10): 951-4. 10.4065/70.10.951.View ArticlePubMedGoogle Scholar
- Le Marchand L, Lum-Jones A, Saltzman B, Visaya V, Nomura AM, Kolonel LN: Feasibility of collecting buccal cell DNA by mail in a cohort study. Cancer Epidemiol Biomarkers Prev. 2001, 10 (6): 701-3.PubMedGoogle Scholar
- Richards B, Skoletsky J, Shuber AP, Balfour R, Stern RC, Dorkin HL, Parad RB, Witt D, Klinger KW: Multiplex PCR amplification from the CFTR gene using DNA prepared from buccal brushes/swabs. Hum Mol Genet. 1993, 2 (2): 159-63. 10.1093/hmg/2.2.159.View ArticlePubMedGoogle Scholar
- Walker AH, Najarian D, White DL, Jaffe JF, Kanetsky PA, Rebbeck TR: Collection of genomic DNA by buccal swabs for polymerase chain reaction-based biomarker assays. Environ Health Perspect. 1999, 107 (7): 517-20. 10.1289/ehp.99107517.View ArticlePubMedPubMed CentralGoogle Scholar
- Walsh DJ, Corey AC, Cotton RW, Foreman L, Herrin GL, Word CJ, Garner DD: Isolation of deoxyribonucleic acid (DNA) from saliva and forensic science samples containing saliva. J Forensic Sci. 1992, 37 (2): 387-95.View ArticlePubMedGoogle Scholar
- Garcia-Closas M, Egan KM, Abruzzo J, Newcomb PA, Titus-Ernstoff L, Franklin T, Bender PK, Beck JC, Le Marchand L, Lum A, Alavanja M, Hayes RB, Rutter J, Buetow K, Brinton LA, Rothman N: Collection of genomic DNA from adults in epidemiological studies by buccal cytobrush and mouthwash. Cancer Epidemiol Biomarkers Prev. 2001, 10 (6): 687-96.PubMedGoogle Scholar
- Birnboim HC, Iwasiow RM, James CMP: Human Genomic DNA Content of Saliva Samples Collected in Oragene®DNA. 2008, Ottawa, Ontario, Canada: DNA Genotek, Inc.Google Scholar
- Feigelson HS, Rodriguez C, Welch R, Hutchinson A, Shao W, Jacobs K, Diver WR, Calle EE, Thun MJ, Hunter DJ, Thomas G, Chanock S: Successful genome-wide scan in paired blood and buccal samples. Cancer Epidemiol Biomarkers Prev. 2007, 16 (5): 1023-5. 10.1158/1055-9965.EPI-06-0859.View ArticlePubMedGoogle Scholar
- Bahlo M, Stankovich J, Danoy P, Hickey PF, Taylor BV, Browning SR, Brown MA, Rubio JP, The Australian and New Zealand Multiple Sclerosis Genetics Consortium (ANZgene): Saliva-Derived DNA Performs Well in Large-Scale, High-Density Single-Nucleotide Polymorphism Microarray Studies. Cancer Epidemiol Biomarkers Prev. 2010, 19: 794-798. 10.1158/1055-9965.EPI-09-0812.View ArticlePubMedGoogle Scholar
- Yokoyama JS, Erdman CA, Hamilton SP: Array-based whole-genome survey of dog saliva DNA yields high quality SNP data. PLoS One. 2010, 5 (5): e10809-10.1371/journal.pone.0010809.View ArticlePubMedPubMed CentralGoogle Scholar
- Accessed at National Cancer Research Institute conference website. http://www.ncri.org.uk/ncriconference/abstract/pdf/2007%20pdfs/B26.pdf.
- Poole CJ, Earl HM, Hiller L, Dunn JA, Bathers S, Grieve RJ, Spooner DA, Agrawal RK, Fernando IN, Brunt AM, O'Reilly SM, Crawford MS, Rea DW, Simmonds P, Mansi JL, Stanley A, Harvey P, McAdam K, Foster L, Leonard RCF, Twelves CJ, NEAT Investigators SCTBG: Epirubicin and Cyclophosphamide, Methotrexate, and Fluorouracil as Adjuvant Therapy for Early Breast Cancer. N Eng J Med. 2006, 355 (18): 1851-62. 10.1056/NEJMoa052084.View ArticleGoogle Scholar
- Wardley AM, Hiller L, Howard HC, Dunn JA, Bowman A, Coleman RE, Fernando IN, Ritchie DM, Earl HM, Poole CJ: tAnGo: a randomised phase III trial of gemcitabine in paclitaxel-containing, epirubicin/cyclophosphamide-based, adjuvant chemotherapy for early breast cancer: a prospective pulmonary, cardiac and hepatic function evaluation. Br J Cancer. 2008, 99 (4): 597-603. 10.1038/sj.bjc.6604538.View ArticlePubMedPubMed CentralGoogle Scholar
- Accessed at DNA Genotek website. http://www.dnagenotek.com.
- Fan JB, Gunderson KL, Bibikova M, Yeakley JM, Chen J, Garcia EW, Lebruska LL, Laurent M, Shen R, Barker D: Illumina universal bead arrays. Methods Enzymol. 2006, 410: 57-73.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1755-8794/5/19/prepub
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