Rare and potentially pathogenic variants in hydroxycarboxylic acid receptor genes identified in breast cancer cases
BMC Medical Genomics volume 14, Article number: 284 (2021)
Three genes clustered together on chromosome 12 comprise a group of hydroxycarboxylic acid receptors (HCARs): HCAR1, HCAR2, and HCAR3. These paralogous genes encode different G-protein coupled receptors responsible for detecting glycolytic metabolites and controlling fatty acid oxidation. Though better known for regulating lipid metabolism in adipocytes, more recently, HCARs have been functionally associated with breast cancer proliferation/survival; HCAR2 has been described as a tumor suppressor and HCAR1 and HCAR3 as oncogenes. Thus, we sought to identify germline variants in HCAR1, HCAR2, and HCAR3 that could potentially be associated with breast cancer risk.
Two different cohorts of breast cancer cases were investigated, the Alabama Hereditary Cancer Cohort and The Cancer Genome Atlas, which were analyzed through nested PCRs/Sanger sequencing and whole-exome sequencing, respectively. All datasets were screened for rare, non-synonymous coding variants.
Variants were identified in both breast cancer cohorts, some of which appeared to be associated with breast cancer BC risk, including HCAR1 c.58C > G (p.P20A), HCAR2 c.424C > T (p.R142W), HCAR2 c.517_518delinsAC (p.G173T), HCAR2 c.1036A > G (p.M346V), HCAR2 c.1086_1090del (p.P363Nfs*26), HCAR3 c.560G > A (p.R187Q), and HCAR3 c.1117delC (p.Q373Kfs*82). Additionally, HCAR2 c.515C > T (p.S172L), a previously identified loss-of-function variant, was identified.
Due to the important role of HCARs in breast cancer, it is vital to understand how these genetic variants play a role in breast cancer risk and proliferation and their consequences on treatment strategies. Additional studies will be needed to validate these findings. Nevertheless, the identification of these potentially pathogenic variants supports the need to investigate their functional consequences.
There are three known genes clustered together on chromosome 12 that comprise a group of hydroxycarboxylic acid receptors (HCARs), HCAR1, HCAR2, and HCAR3. With extreme homology, these paralogous genes encode three different heterotrimeric G-protein coupled receptors (GPCRs), commonly referred to as GPR81, GPR109A, and GPR109B, respectively. These genes are responsible for detecting glycolytic metabolites and controlling the rate of fatty acid oxidation [1, 2]. GPCRs are characterized by 7-transmembrane domains, an N-terminus that interacts with extracellular components, and a C-terminus responsible for transmitting intracellular signals. Since GPCRs are embedded in the cellular membrane and initiate signal transduction, they eliminate the challenge of intracellular drug administration; thus, they are very effective treatment options targeted by 30–50% of existing pharmaceuticals .
Though better known for their regulation of lipid metabolism in adipocytes, more recently, HCARs have been functionally associated with breast cancer (BC) [1, 2, 4,5,6,7]. For instance, HCAR2 is described as a tumor suppressor gene because it exhibits a 70% reduction of cell-surface expression in primary BC cells; and its cellular expression is essential for the initiation of apoptosis by its endogenous ligands . In contrast, HCAR1 and HCAR3 are considered oncogenes that show notably increased mRNA expressions in BC cells compared to controls and result in BC cell death when knocked down [5, 6]. Knock-down of HCAR3 has been demonstrated to result in BC cell death through uncontrolled up-regulation of fatty acid oxidation, which can be mitigated by introducing fatty acid oxidation inhibitors . Furthermore, upon activation by lactate or butyrate, HCAR1 increases the expression of DNA repair proteins and subsequently increases Hela cells' resistance to doxorubicin . Doxorubicin is a common BC chemotherapy drug. Reduced doxorubicin sensitivity is observed during BC treatment, potentially mediated by extracellular matrix proteins ; thus, HCAR1 could be contributing to the resistance.
The metabolic function, differential expression, and promising therapeutic potential of HCARs make their involvement in BC tumorigenesis an exciting research topic. With potential implications in cancer precision medicine, it is essential to identify inherited genetic variants associated with BC risk, molecular subtype, and drug metabolism. This manuscript details the genetic screening of HCAR1, HCAR2, and HCAR3 in 46 BC cases from the Alabama Hereditary Cancer Cohort (AHCC) and 649 BC cases from The Cancer Genome Atlas (TCGA)  to identify rare, inherited variants with potentially damaging effects.
Alabama Hereditary Cancer Cohort (AHCC)
Forty-six unrelated BC cases from the AHCC, who self-described as being White/European American, enrolled in a hereditary cancer genetics research study through Auburn University Institutional Review Board-approved protocols, 14–232 or 15–111, which was previously described by Bishop et al. . Informed consent was obtained through writing for all study participants. In brief, the study criteria included BC-affected individuals with a family history of the disease or diagnosed with BC under 45 years. Blood samples were obtained from all participants, and genomic DNA was extracted for genetic analyses.
AHCC genetic analyses
Due to the extreme homology between HCAR1 (NM_032554.3), HCAR2 (NM_177551.3), and HCAR3 (NM_006018.2), primers were carefully designed using Primer3Plus to amplify each gene separately through nested PCRs (Additional file 1: Tables S1, S2, and S3 and Figures S1, S2, and S3). External primers were designed for each gene that initially amplified a large (> 3.1 Kb) PCR product from genomic DNA. The forward and reverse external primers were located upstream and downstream of each transcript and uniquely hit once in the genome according to the University of California, Santa Cruz (UCSC) BLAT [10, 11]. Overall, this external primer design increased specificity and avoided amplification of the other paralogous genes. Internal primers were designed to amplify smaller regions of each large amplicon through nested PCRs. For this study, which screened for coding variants in each gene, only internal primers that targeted coding regions were used for mutation analysis (Additional file 1: Tables S1, S2, and S3 and Figures S1, S2, and S3). Touchdown PCRs were carried out using a final annealing temperature of 56 °C. Extension times varied according to amplicon size (1 min/Kb), and betaine was required for GC rich amplicons. Nested PCR amplicons were Sanger sequenced at Eurofins Genomics. Sequences were analyzed using Mutation Surveyor (Soft Genetics). Variant validation followed a similar process as previously described , and only rare and non-synonymous coding variants (with European Americans MAFs of < 1% in Exome Variant Server (EVS) ) were validated. EVS provides whole-exome sequencing data in aggregate for ~ 4300 individuals, providing ~ 8600 control alleles for assessment.
Some internal primer sets would have amplified multiple products if the template was genomic DNA. To demonstrate that nested PCRs increased specificity, we used UCSC In-silico PCR (hg38 assembly) to determine genomic DNA amplicons  and BLASTn to align/compare those amplicons [14, 15]. Electropherograms of our nested PCR products confirmed proper amplification by viewing the specific positions that varied between the predicted/in-silico amplicons, representing HCAR1, HCAR2, or HCAR3 (i.e., Additional file 1: Figure S4, confirming HCAR3 c.560G > A (p.R187Q)).
In R (v 3.5.1), each validated variant was investigated using Fisher's exact test [16, 17] to generate p values comparing allele frequencies between AHCC BC cases and EVS ethnic-matched controls. p Values of < 0.05 were considered to be statistically significant and were not corrected for multiple testing. In addition, the pathogenicity of each missense variant was predicted using Polyphen2 , and amino acid conservation was assessed using WebLogo . Finally, regarding frameshift variants, Mutalyzer , Phyre2 , I-TASSER [22, 23], and PSORT [24, 25] were used to determine differences between the wildtype and mutant protein.
The Cancer Genome Atlas (TCGA)
For the identification of germline variants, a total of 649 blood-derived whole-exome binary sequence alignment mapping (BAM) files were downloaded through the approved research project #10805 using the Genomic Data Commons (GDC) Data Portal Repository. All individuals were categorized as European American or "White" BC cases. For sample acquisition, specific filters in the 'Cases’ category included: Project (TCGA-BRCA), Sample Type (Blood-Derived Normal (NB)), and Race (‘White’). Samples were filtered further in the ‘Files’ category and included Experimental Strategy (WXS) and Data Format (BAM). These files were downloaded using the GDC Data Transfer Tool (version 1.2.0). A total of 650 sample files were obtained for European Americans. Only individuals with known ages of BC onset were included in this study; therefore, one European American BAM file was removed from all subsequent analyses. The 649 BAM files had been previously aligned to the hg38 human reference genome. Upon download, the files were processed using a bioinformatics pipeline adapted from the Genome Analysis Toolkit’s (GATK’s) best practices pipeline. HCAR1 (NM_032554; chr12:122726076-122730844), HCAR2 (NM_177551; chr12:122701293-122703357), and HCAR3 (NM_006018; chr12:122714756-122716811) were extracted from the variant calling format (VCF) files and then annotated using ANNOVAR (version June2017). Variants were filtered to include rare, non-synonymous variants with ethnic-specific minor allele frequencies of < 1% in EVS .
Variants (and surrounding nucleotides within the same reads) were visualized using recalibrated BAM files in Integrative Genomic Viewer (IGV) to determine validity and confirm proper alignment, considering the extreme homology between the HCAR genes, particularly HCAR2 and HCAR3 (Additional file 1: Figures S5, S6, and S7). BLASTn was used to align HCAR2 and HCAR3 nucleotide/mRNA sequences to note the sequence differences, aiding in alignment confirmation [14, 15]. For each visually confirmed variant, Fisher’s exact tests were performed to compare allele frequencies in TCGA BC cases versus controls (EVS; European Americans) , similar to the above described AHCC individual variant statistical analysis. Additionally, the Fisher method was used for gene-based aggregation analyses for TCGA data; the ‘sumlog’ command was used as part of the ‘metap’ packages in R [26, 27].
A total of four rare, non-synonymous variants were identified in four different BC cases from the AHCC, two in HCAR1 and two in HCAR3 (Table 1 and Supplementary information: Table S4). In HCAR1, two highly conserved and probably damaging missense variants, HCAR1 c.58C > G (p.P20A) and HCAR1 c.721C > T (p.L241F), were identified (Table 1, and Figs. 1, 2A). HCAR1 c.58C > G (p.P20A) had a statistically significant difference in allele frequencies between cases and controls, suggesting an association with BC risk (Table 1). Both HCAR3 variants, HCAR3 c.560G > A (p.R187Q) and HCAR3 c.1117delC (p.Q373Kfs*82), also appeared to be associated with BC (Table 1). However, HCAR3 c.560G > A (p.R187Q), located in an extracellular loop, was predicted to be benign (Table 1, Figs. 1, 2C, and Additional file 1: Figure S4). HCAR3 c.1117delC (p.Q373Kfs*82) greatly extends the C-terminus of HCAR3 and changes the secondary and tertiary protein structure (Fig. 3). Moreover, PSORT predicted that the mutant HCAR3 p.Q373Kfs*82 protein loses a prenylation motif and gains both an ER Membrane Retention Signal and a peroxisomal targeting signal (Table 2).
Subsequent analysis of 649 BC cases in TCGA identified numerous rare, non-synonymous variants in HCAR1, HCAR2, and HCAR3 (Table 3 and Fig. 2). No variants in HCAR1 or HCAR3 were associated with BC risk, including HCAR1 c.721C > T (p.L241F), which was also detected in the AHCC, as well as other probably damaging variants (Table 3). Four BC-associated variants in HCAR2 were identified, including one frameshift, HCAR2 c.1086_1090del (p.P363Nfs*26) (Table 3 and Fig. 4), and three missense variants, HCAR2 c.424C > T (p.R142W), HCAR2 c.517_518delinsAC (p.G173T), and HCAR2 c.1036A > G (p.M346V) (Table 3 and Figs. 2B). Aggregation analyses did not reveal any gene-based associations (Table 3 and Supplementary Information: Table S5).
Upon screening 46 European American BC cases from the AHCC for rare, non-synonymous variants in HCAR1, HCAR2, and HCAR3, a total of four variants were identified in four different BC cases. These variants were exclusively identified in HCAR1 and HCAR3, which is notable considering their suggested oncogenic role and requirement for BC proliferation/survival compared to the demonstrated tumor suppressor properties of HCAR2 [4,5,6]. Subsequent screening of a larger cohort, 649 European American BC cases from TCGA, identified numerous rare, non-synonymous variants in HCAR1, HCAR2, and HCAR3, but only variants in HCAR2 were associated with BC risk. No gene-based associations were identified.
HCAR2, the nicotinic acid receptor, has recently been reported to have BC tumor suppressing properties [4, 5]; thus, rare, non-synonymous variants in HCAR2 likely disrupt protein function, increasing BC susceptibility. No HCAR2 variants were detected in the AHCC. This was likely due to the small cohort size and the rarity of such variants. Nine different HCAR2 variants were detected in the TCGA BC cohort, four of which generated statistically significant p-values. One statistically significant variant was HCAR2 c.1086_1090del (p.P363Nfs*26). This five base pair deletion affects one of the few differences between the nucleotide sequences of HCAR2 and HCAR3 (Fig. 4). Typically, HCAR2 has those five nucleotides, which HCAR3 lacks. Because of this extreme homology and the possibility of misalignment of HCAR3 reads, variant confirmation was pertinent. Ultimately, this variant and all other reported HCAR2 variants were confirmed through visualization in IGV and the comparison of proximate HCAR2/3 differences within the same mutation-harboring reads (Table 3 and Additional file 1: Figure S5). This process also eliminated variants that were a result of misalignment (Additional file 1: Figure S7). Ultimately, the HCAR2 p.P363Nfs*26 mutant protein was predicted to gain 25 amino acids at its C-terminus, mimicking the C-terminus of HCAR3 (Fig. 4). This conversion could explain the variant’s pathogenicity since the HCAR2 C-terminus is essential for normal function , and HCAR3 has BC oncogenic properties .
Several HCAR2 residues have been previously demonstrated to be critical for protein function (Fig. 2B) [28,29,30]. Tunaru et al. reported N86, W91, R111, S178, F276, and Y284 as critical for nicotinic acid binding . Yasuda et al. identified an atypical motif, N17-C18-C19, crucial for normal surface trafficking and other residues in extracellular regions, C100, C177, C183, and C266, essential for HCAR2 activation . Furthermore, Li et al. discovered a sequence of residues (329–343) in the C-terminus critical in keeping HCAR2 in an inactive conformation . Our study did not identify HCAR2 missense variants that specifically affected the aforementioned critical residues, but some identified variants were in close proximity (Fig. 2B). Interestingly, most of the detected HCAR2 missense variants were predicted to be benign, including the three BC-associated missense variants, HCAR2 c.424C > T (p.R142W), HCAR2 c.517_518delinsAC (p.G173T), and HCAR2 c.1036A > G (p.M346V). Similar to HCAR2 c.1086_1090del (p.P363Nfs*26), both HCAR2 c.424C > T (p.R142W) and HCAR2 c.517_518delinsAC (p.G173T) affect nucleotides that differ between HCAR2 and HCAR3, yet they too were confirmed as true variant calls (Additional file 1: Figure S5). These missense variants could convert HCAR2’s function since they result in residues specific to HCAR3. HCAR2 c.424C > T (p.R142W) is located at a transmembrane/cytoplasmic loop junction. Notably, several critical residues in HCAR2 [29, 30] and HCAR1 [31, 32] are at or near such junctions (Fig. 2A, B).
The HCAR1 protein is known as the lactate receptor, and, upon HCAR1 binding, lactate inhibits lipolysis [1, 2, 33]. Like HCAR2, many residues have been deemed critical for HCAR1 function (Fig. 2A) [31,32,33]. Most notably, HCAR1 c.515C > T (p.S172L), a loss-of-function variant identified by Doyle et al. , was detected in six BC cases in the TCGA cohort. Interestingly, PolyPhen2 analysis predicted the variant to be benign, demonstrating the potential inaccuracies of such prediction software . Furthermore, despite being detected at a higher frequency in BC cases than controls, the difference was not statistically significant; thus, additional studies are required to determine if this loss-of-function variant is associated with BC risk. Regarding other critical residues, Liu et al. demonstrated that particular missense variants in transmembrane domains three (p.R99A), six (p.Y233A and p.R240A), and seven (p.T267A) diminished the response of HCAR1 to L-lactate (Fig. 2A) . It is important to note the proximity of p.R240A to another missense variant detected in this study, p.L241F, which is at a transmembrane and extracellular domain junction (Fig. 2A). The vital function of R240 in lactate binding, as well as the identification of other critical residues located at or near similar junctions (Fig. 2A) [31, 32], hints towards the importance of the highly conserved L241 and the potentially damaging effects of an amino acid substitution at that location. Interestingly, HCAR1 p.L241F was the only variant detected in both BC cohorts (AHCC and TCGA) in this study. Despite not appearing to be associated with BC, it is a rare allele that is predicted to be damaging. Coincidentally, two other HCAR1 missense variants detected in this study, HCAR1 c.58C > G (p.P20A) and HCAR1 c.334G > A (p.D112N), are also near a transmembrane and extracellular/cytoplasmic domain junction (Fig. 2A). Furthermore, HCAR1 p.P20A is in the N-terminus close to two other critical cysteine residues, C6 and C7 (Fig. 2A) . The N-terminus is essential for ligand binding, dimerization, signaling, and surface expression (31, 35,36,37). Therefore, HCAR1 p.P20A is likely a functional variant. It was also determined to be associated with BC in the AHCC cohort.
Considering that five of the seven detected HCAR1 missense variants are predicted to be pathogenic, determining their functional involvement in BC is critical. To date, all functionally assessed HCAR1 variants have been deemed loss-of-function (highlighted in color in Fig. 2A) [31,32,33]. However, with HCAR1 typically being regarded as critical for BC proliferation and survival by controlling lipid/fatty acid metabolism [5, 6], a loss-of-function mutation would presumably result in BC cell death. Interestingly, knocking down HCAR1 has different effects on different BC molecular subtypes [5, 6]. For instance, knocking down HCAR1 in a HER2-enriched BC cell line, HCC1954 (ER-, PR-, HER2 +), and triple-negative BC cell line, HCC38 (ER-, PR-, HER2-), resulted in a significant decrease of cell viability within 48 h of transfection. However, there was no significant change in viability regarding the luminal B cell line, BT-474 (ER + , PR + , HER2 +), similar to the non-tumorigenic epithelial breast cell line, MCF12A . Furthermore, when HCAR1 was knocked down in the luminal A BC cell lines, MCF-7 and T47D, cell viability decreased . In this study, the BC molecular subtype was available for most BC cases in the AHCC; thus, we confirmed that the individuals in the AHCC with HCAR1 p.P20A and HCAR1 p.L241F were diagnosed with luminal subtypes. Specifically, the individual with HCAR1 p.P20A had luminal A BC (ER + and HER2-), which according to Lee et al. requires HCAR1 to proliferate . The individual with HCAR1 p.L241F was ER + and PR + , but HER2 status was unknown; thus, the subtype could not be confirmed as luminal A or B. If it were luminal B, HCAR1 expression would not be not required for survival , whereas it would be necessary for luminal A . In addition to knock-down studies, Lee et al. investigated HCAR1 expression levels in different BC molecular subtypes and noted that ER + BC cell lines expressed HCAR1 at a higher level ; this ER + BC-association has also been reported for another GPCR, GPR30 . Thus, it is important to determine if HCAR1 missense variants are specifically associated with ER + BC, as well as if they are loss- or gain-of-function mutations. Unfortunately, the sample size of the AHCC was too small to determine subtype-specific associations, and subtype information was not provided in the clinical information of the TCGA dataset.
HCAR3, which is only found in higher primates, is the receptor for 3-hydroxylated β-oxidation intermediates, particularly 3-hydroxy-octanoate [1, 2]. When activated, HCAR3 inhibits free fatty acid release from cells, providing a negative feedback mechanism to offset stimuli that promote lipolysis and fatty acid oxidation. Knocking down HCAR3 in BC cell lines BT-474, HCC1954, and HCC38 induced cell death, suggesting that HCAR3 has oncogenic properties. Introducing fatty acid oxidation inhibitors mitigated the knock-down effects, confirming that uncontrolled up-regulation of fatty acid oxidation promotes BC cell death; thus, HCAR3 plays a vital role in controlling fatty acid metabolism in BC cells . Accordingly, one can presume that BC-associated HCAR3 variants have gain-of-function effects. However, HCAR3 knock-down effects have not been assessed in luminal A BC cell lines, which is the molecular subtype reported in the two BC-affected individuals from the AHCC with HCAR3 BC-associated variants, HCAR3 c.560G > A (p.R187Q) and HCAR3 c.1117delC (p.Q373Kfs*82). Numerous HCAR3 genetic variants have been reported in publically available databases [13, 39], as well as through polymerase chain reaction (PCR)-based techniques . While their pathogenic effects have not been functionally assessed, screening results of the AHCC suggested that rare, non-synonymous variants in HCAR3 may enhance the receptor’s ability to control fatty acid metabolism. Nonetheless, three HCAR3 missense variants detected in BC cases in TCGA did not appear to be associated with BC risk. Additionally, HCAR3 p.R187Q was predicted to be benign. Even though prediction software have been shown to misclassify known pathogenic variants , it is important to note that HCAR2 has a glutamine at that overlapping position. Though we have confirmed HCAR3 p.R187Q through nested PCR (Additional file 1: Figure S4), it is unknown if this change would affect the function of HCAR3. That being said, with such slight differences between the two proteins, perhaps each alteration is key to protein function. Incidentally, this variant was detected in an early onset BC case also determined to harbor a clinically significant frameshift mutation in NBN [12, 41]. The interaction of these two variants and their combined ability to promote BC is unknown, but, intriguingly, expression of both HCAR3 and NBN have been reported to be dysregulated in the oocytes of older women, when investigating why aneuploidy pregnancies occur in women of older ages. Overall, this observation suggests that these genes may play a role in proper chromosome segregation and maintaining genomic integrity, which is a phenomenon also disrupted in cancer [42, 43].
The HCAR3 frameshift mutation, HCAR3 p.Q373Kfs*82, significantly extends the C-terminus cytoplasmic tail of the mutant HCAR3 protein and changes the secondary and tertiary protein structure (Fig. 3). Again, based on the suggested oncogenic role of HCAR3 in BC, HCAR3 p.Q373Kfs*82 may potentially result in a gain-of-function. Interestingly, distinct mutation profiles, corresponding to clusters of nonsense and frameshift mutations in the C-termini of GPCRs, GPR34, CCR6, and CCR4, have been reported in mucosa-associated lymphoid tissue (MALT) lymphoma and adult T cell leukemia/lymphoma (ATLL) as gain-of-function mutations [44,45,46]. Even though the nonsense and frameshift mutations reported in GPR34, CCR6, and CCR4 truncate the encoded proteins, PSORT predicted that HCAR3 p.Q373Kfs*82 abolishes a prenylation motif. Similarly, the GPR34 mutations eliminate a key phosphorylation motif and ultimately dysregulate the receptor’s desensitization process [44, 45]. Additionally, the mutant HCAR3 protein gains an ER Membrane Retention Signal, potentially affecting internalization patterns, which is also disrupted with CCR4 gain-of-function mutations [44, 46]. Contrarily, the gain of a predicted peroxisomal targeting signal to the mutant HCAR3 hints toward protein degradation, a loss-of-function mechanism. On a similar note, read-through mutations that result in mutant proteins with C-terminal extensions in PNPO and HSD3B2 cause hereditary disorders through protein degradation . Nonetheless, GATA3 frameshift mutations that extend the C-terminus are the most common somatic mutation identified in TCGA BC patients and display gain-of-function activity . In addition, loss-of-function GATA3 mutations were also identified, demonstrating that both loss- and gain-of-function mutations can be identified in the same gene and associated with BC. Similarly, TP53, a clinically valid BC susceptibility gene, has both tumor suppressor and oncogenic properties [49,50,51]. Thus, the exact functional consequences of HCAR3 p.Q373Kfs*82 may be complex but are important to elucidate, especially considering the vital functions of the C-terminus of HCAR proteins .
HCAR1, HCAR2, and HCAR3 are three genes clustered on chromosome 12 that encode HCARs, known GPCRs that play a critical role in lipid metabolism, even in the context of BC proliferation and survival. Upon genetic analysis of two cohorts of BC cases, potentially damaging, non-synonymous genetic variants in HCAR1, HCAR2, and HCAR3 were identified that could alter receptor function. Though no gene-based associations were revealed, the identification of individual variant associations supports the need to investigate the functional consequences of these variants. However, these genetic associations need to be validated in future studies. Ultimately, it is vital to understand how these genetic variants play a role in BC risk and proliferation and their consequences on treatment strategies, particularly regarding the use of doxorubicin, a commonly prescribed BC chemotherapy drug [7, 8].
Availability of data and materials
Sanger sequencing data of HCAR breast cancer mutations are available through two different respositories; fasta files can be obtained through the NCBI SRA repository through BioProject PRJNA778760 (https://www.ncbi.nlm.nih.gov/sra/PRJNA778760); ab1 files can be obtained through the Auburn University Scholarly Repository (Aurora; https://doi.org/10.35099/aurora-83). TCGA whole-exome sequencing data analyzed during this study are available for download through the NCI GDC Data Portal (https://portal.gdc.cancer.gov). See methods for specific repository filters used for sample acquisition from the TCGA-BRCA project.
Alabama Hereditary Cancer Cohort
Adult T cell leukemia/lymphoma
Binary sequence alignment mapping
Ductal carcinoma in situ
Exome Variant Server
Genomic Data Commons
G-protein coupled receptor
Hydroxycarboxylic acid receptor
Human epidermal growth factor receptor 2
Integrative Genomic Viewer
Minor allele frequency
Mucosa-associated lymphoid tissue
Polymerase chain reactions
Sorting intolerant from tolerant
The Cancer Genome Atlas
Variant calling format
Ahmed K. Biological roles and therapeutic potential of hydroxy-carboxylic Acid receptors. Front Endocrinol (Lausanne). 2011;2:51.
Offermanns S. Hydroxy-carboxylic acid receptor actions in metabolism. Trends Endocrinol Metab. 2017;28(3):227–36.
Fang Y, Kenakin T, Liu C. Editorial: orphan GPCRs as emerging drug targets. Front Pharmacol. 2015;6:295.
Elangovan S, Pathania R, Ramachandran S, Ananth S, Padia RN, Lan L, et al. The niacin/butyrate receptor GPR109A suppresses mammary tumorigenesis by inhibiting cell survival. Cancer Res. 2014;74(4):1166–78.
Staubert C, Broom OJ, Nordstrom A. Hydroxycarboxylic acid receptors are essential for breast cancer cells to control their lipid/fatty acid metabolism. Oncotarget. 2015;6(23):19706–20.
Lee YJ, Shin KJ, Park SA, Park KS, Park S, Heo K, et al. G-protein-coupled receptor 81 promotes a malignant phenotype in breast cancer through angiogenic factor secretion. Oncotarget. 2016;7(43):70898–911.
Wagner W, Kania KD, Blauz A, Ciszewski WM. The lactate receptor (HCAR1/GPR81) contributes to doxorubicin chemoresistance via ABCB1 transporter up-regulation in human cervical cancer HeLa cells. J Physiol Pharmacol. 2017;68(4):555–64.
Lovitt CJ, Shelper TB, Avery VM. Doxorubicin resistance in breast cancer cells is mediated by extracellular matrix proteins. BMC Cancer. 2018;18(1):41.
Bishop MR, Shah A, Shively M, Huskey ALW, Omeler SM, Bilgili EP, et al. Establishment of the Alabama Hereditary Cancer Cohort - strategies for the inclusion of underrepresented populations in cancer genetics research. Mol Genet Genomic Med. 2018;6(5):766–78.
Kent WJ. BLAT–the BLAST-like alignment tool. Genome Res. 2002;12(4):656–64.
Kent WJ, Sugnet CW, Furey TS, Roskin KM, Pringle TH, Zahler AM, et al. The human genome browser at UCSC. Genome Res. 2002;12(6):996–1006.
Bishop MR, Huskey ALW, Hetzel J, Merner ND. A research-based gene panel to investigate breast, ovarian and prostate cancer genetic risk. PLoS ONE. 2019;14(8):e0220929.
Exome Variant Server. NHLBI GO Exome Sequencing Project (ESP) Seattle, WA2019. http://evs.gs.washington.edu/EVS/.
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;215(3):403–10.
McGinnis S, Madden TL. BLAST: at the core of a powerful and diverse set of sequence analysis tools. Nucleic Acids Res. 2004;32(Web Server issue):W20-5.
Sprent P. Fisher exact test. In: Lovric M, editor. International encyclopedia of statistical science. Berlin: Springer; 2011. p. 524–5.
Gilliam D, O’Brien DP, Coates JR, Johnson GS, Johnson GC, Mhlanga-Mutangadura T, et al. A homozygous KCNJ10 mutation in Jack Russell Terriers and related breeds with spinocerebellar ataxia with myokymia, seizures, or both. J Vet Intern Med. 2014;28(3):871–7.
Adzhubei I, Jordan DM, Sunyaev SR. Predicting functional effect of human missense mutations using PolyPhen-2. Curr Protoc Hum Genet. 2013;Chapter 7:Unit7 20.
Crooks GE, Hon G, Chandonia JM, Brenner SE. WebLogo: a sequence logo generator. Genome Res. 2004;14(6):1188–90.
Wildeman M, van Ophuizen E, den Dunnen JT, Taschner PE. Improving sequence variant descriptions in mutation databases and literature using the Mutalyzer sequence variation nomenclature checker. Hum Mutat. 2008;29(1):6–13.
Kelley LA, Mezulis S, Yates CM, Wass MN, Sternberg MJ. The Phyre2 web portal for protein modeling, prediction and analysis. Nat Protoc. 2015;10(6):845–58.
Roy A, Kucukural A, Zhang Y. I-TASSER: a unified platform for automated protein structure and function prediction. Nat Protoc. 2010;5(4):725–38.
Yang J, Zhang Y. I-TASSER server: new development for protein structure and function predictions. Nucleic Acids Res. 2015;43(W1):W174–81.
Nakai K, Horton P. PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization. Trends Biochem Sci. 1999;24(1):34–6.
Horton P, Park KJ, Obayashi T, Fujita N, Harada H, Adams-Collier CJ, et al. WoLF PSORT: protein localization predictor. Nucleic Acids Res. 2007;35(Web Server issue):W585-7.
Fisher RA. Statistical methods for research workers. Edinburgh: Oliver and Boyd; 1925.
Sutton AJ, Abrams KR, Jones DR, Sheldon TA, Song F. Methods for meta-analysis in medical research. Chichester: Wiley; 2000.
Li G, Zhou Q, Yu Y, Chen L, Shi Y, Luo J, et al. Identification and characterization of distinct C-terminal domains of the human hydroxycarboxylic acid receptor-2 that are essential for receptor export, constitutive activity, desensitization, and internalization. Mol Pharmacol. 2012;82(6):1150–61.
Tunaru S, Lattig J, Kero J, Krause G, Offermanns S. Characterization of determinants of ligand binding to the nicotinic acid receptor GPR109A (HM74A/PUMA-G). Mol Pharmacol. 2005;68(5):1271–80.
Yasuda D, Imura Y, Ishii S, Shimizu T, Nakamura M. The atypical N-glycosylation motif, Asn-Cys-Cys, in human GPR109A is required for normal cell surface expression and intracellular signaling. FASEB J. 2015;29(6):2412–22.
Kuei C, Yu J, Zhu J, Wu J, Zhang L, Shih A, et al. Study of GPR81, the lactate receptor, from distant species identifies residues and motifs critical for GPR81 functions. Mol Pharmacol. 2011;80(5):848–58.
Doyle JR, Lane JM, Beinborn M, Kopin AS. Naturally occurring HCA1 missense mutations result in loss of function: potential impact on lipid deposition. J Lipid Res. 2013;54(3):823–30.
Liu C, Wu J, Zhu J, Kuei C, Yu J, Shelton J, et al. Lactate inhibits lipolysis in fat cells through activation of an orphan G-protein-coupled receptor, GPR81. J Biol Chem. 2009;284(5):2811–22.
Thusberg J, Olatubosun A, Vihinen M. Performance of mutation pathogenicity prediction methods on missense variants. Hum Mutat. 2011;32(4):358–68.
Lindner D, Walther C, Tennemann A, Beck-Sickinger AG. Functional role of the extracellular N-terminal domain of neuropeptide Y subfamily receptors in membrane integration and agonist-stimulated internalization. Cell Signal. 2009;21(1):61–8.
Uddin MS, Kim H, Deyo A, Naider F, Becker JM. Identification of residues involved in homodimer formation located within a beta-strand region of the N-terminus of a Yeast G protein-coupled receptor. J Recept Signal Transduct Res. 2012;32(2):65–75.
Uddin MS, Hauser M, Naider F, Becker JM. The N-terminus of the yeast G protein-coupled receptor Ste2p plays critical roles in surface expression, signaling, and negative regulation. Biochim Biophys Acta. 2016;1858(4):715–24.
Carmeci C, Thompson DA, Ring HZ, Francke U, Weigel RJ. Identification of a gene (GPR30) with homology to the G-protein-coupled receptor superfamily associated with estrogen receptor expression in breast cancer. Genomics. 1997;45(3):607–17.
Lek M, Karczewski KJ, Minikel EV, Samocha KE, Banks E, Fennell T, et al. Analysis of protein-coding genetic variation in 60,706 humans. Nature. 2016;536(7616):285–91.
Zellner C, Pullinger CR, Aouizerat BE, Frost PH, Kwok PY, Malloy MJ, et al. Variations in human HM74 (GPR109B) and HM74A (GPR109A) niacin receptors. Hum Mutat. 2005;25(1):18–21.
National Comprehensive Cancer Network. Genetic/Familial High-Risk Assessment: Breast and Ovarian (Version 3.2019) 2019. https://www.nccn.org/professionals/physician_gls/pdf/genetics_screening.pdf.
Nik-Zainal S. From genome integrity to cancer. Genome Med. 2019;11(1):4.
Barone S, Sarogni P, Valli R, Pallotta MM, Silvia G, Frattini A, et al. Chromosome missegregation in single human oocytes is related to the age and gene expression profile. Int J Mol Sci. 2020. https://doi.org/10.3390/ijms21061934.
Martinez-Climent JA. G-protein coupled receptor (GPCR) mutations in lymphoid malignancies: linking immune signaling activation and genetic abnormalities. Haematologica. 2018;103(8):1252–5.
Moody S, Thompson JS, Chuang SS, Liu H, Raderer M, Vassiliou G, et al. Novel GPR34 and CCR6 mutation and distinct genetic profiles in MALT lymphomas of different sites. Haematologica. 2018;103(8):1329–36.
Nakagawa M, Schmitz R, Xiao W, Goldman CK, Xu W, Yang Y, et al. Gain-of-function CCR4 mutations in adult T cell leukemia/lymphoma. J Exp Med. 2014;211(13):2497–505.
Shibata N, Ohoka N, Sugaki Y, Onodera C, Inoue M, Sakuraba Y, et al. Degradation of stop codon read-through mutant proteins via the ubiquitin-proteasome system causes hereditary disorders. J Biol Chem. 2015;290(47):28428–37.
Mair B, Konopka T, Kerzendorfer C, Sleiman K, Salic S, Serra V, et al. Gain- and loss-of-function mutations in the breast cancer gene GATA3 result in differential drug sensitivity. PLoS Genet. 2016;12(9):e1006279.
Miller M, Shirole N, Tian R, Pal D, Sordella R. The evolution of TP53 mutations: from loss-of-function to separation-of-function mutants. J Cancer Biol Res. 2016;4(4):1091.
Brosh R, Rotter V. When mutants gain new powers: news from the mutant p53 field. Nat Rev Cancer. 2009;9(10):701–13.
Zhu J, Sammons MA, Donahue G, Dou Z, Vedadi M, Getlik M, et al. Gain-of-function p53 mutants co-opt chromatin pathways to drive cancer growth. Nature. 2015;525(7568):206–11.
Firstly, we would like to thank all of our study participants. Their selfless enrollment into the AHCC enables our research efforts, and we could not be more grateful. We would also like to thank our community and hospital partners who continuously support our research endeavors. Lastly, we would like to acknowledge the Auburn University Office of Information Technology for their High-Performance Computing support.
This research was supported by Dr. Merner’s Auburn University start-up funds, as well as multiple entities that provided student fellowships, including a National Science Foundation Research Excellence for Undergraduates Grant (Award Number: 1560115), the Auburn University Undergraduate Research Fellows Program, the AURIC Graduate Student Fellowship Program, and the Auburn University CMB Peaks of Excellence Research Fellowship Grant (NSF-EPS-1158862, Grant G00006750). The funding bodies played no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.
Ethics approval and consent to participate
All procedures performed in this study involving human participants were performed in accordance with the Declaration of Helsinki. This study was approved by the Auburn University Institutional Review Board of the Office of Research Compliance through protocols 14-232 MR 1410, 15-111 MR 1503,, and 19-302 EP 1907. Informed consent was obtained through writing for all AHCC study participants. Furthermore, a request (#44682–1) for TCGA Data Use Certification was submitted and the project (#10805) was approved.
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McGuire Sams, C., Shepp, K., Pugh, J. et al. Rare and potentially pathogenic variants in hydroxycarboxylic acid receptor genes identified in breast cancer cases. BMC Med Genomics 14, 284 (2021). https://doi.org/10.1186/s12920-021-01126-3